Era7 Bioinformatics has developed brand-new 16S Microbiome analysis methods reinforcing its position as a global leader in Microbiome services



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Era7 Bioinformatics’ research team has designed a new mathematical model for NGS amplicons analysis using new conceptually different strategies for reference sequences indexing and for finding the best taxonomic assignment for each read, based on (ultra)metric spaces and on their topological properties.

The models, techniques, algorithms and ideas, made public in this preprint: are particularly relevant to scenarios akin to 16S taxonomic profiling, where both the number of reference sequences and the read diversity are considerable. Thus, these new theoretical framework and mathematical model have been the foundations of our new 16S bioinformatics software that reinforces Era7's position as a global leader in Microbiome analysis services.

In this new conceptual context it is possible to design with flexibility new experimental approaches for 16S amplicon sequencing in which the variable regions to be sequenced can be non-contiguous. Based on this possibility we have designed a new amplicon to analyze 16S V4 and V6 regions his approach allows the taxonomic assignment at a high level of resolution using an asymmetric design with R1 of 180 bp and R2 of 120 bp. It saves around 50% of the sequence comparing with the classical V3V4 symmetric amplicon design with overlapping R1 and R2 of 300 bp each of them.