HLA amplicons NGS sequencing approaches
Sequencing HLA alleles amplicons is probably the best way to know which allele / alleles are present in an individual and even discover new ones. Depending on the technology used different approaches could be done:
- Short reads technologies like illumina. In this case the best and more secure approach would be the amplification of exons and the subsequent sequencing procedure.
- Using PacBio an approach amplifying whole HLA genes could be done. In this case , it should be considered that of the known HLA alleles sequences are known only for certain exons and not for the entire gene. However, using only exon information is always possible.
NGS technologies used for HLA amplicon sequencing allows the investigation of a large number of samples in a very affordable way.