454 header
 

454


454 was the first NGS platform that appeared in the market.

 

Advantages:

List item Read length

Its main advantage was the length of the reads

Disadvantages:

List item High price compared with yielding
List item Frequent errors in homopolymers
List item Not uniform coverage

As this was the first technology introduced in the market for NGS, in Era7 Bionformatics we have a lot of experience analyzing data from 454 machines:

List item 16S Metagenomics
List item Bacterial Genomics
List item De novo RNA-seq
List item Amplicons

454 platforms :

List item 454 GS Junior.
454 GS Junior.

A personal Sequencer.

See also: http://454.com/products/gs-junior-system/index.asp