RNA-Seq for bacteria: unique insights into bacterial function

RNA-seq is increasingly being used for exploring gene expression in bacteria. Different RNA-seq approaches can be designed to get insights into the transcriptome of a specific clone, a reduced bacterial community or into the meta-transcriptome of a complex bacterial community. A very interesting approach is to do Dual RNA-seq to gain knowledge about Host and Pathogen relationships analyzing the expression of the Pathogen genes in relation with the expression of the Host at different time points.

Our system

    -  Tolerant to errors inherent to next-gen sequencing
    -  It does not need a reference genome
    -  Customized to client specific goals

Turnaround times
    -  One transcriptome in two weeks
    -  Transcriptome Express: one transcriptome in one week!

Non-normalized cDNA libraries

    -  Rich functional annotation (GO, Interpro, references)
    -  Gene expression quantification
    -  Differential expression analysis
    -  De novo characterization of your transcriptome
    -  Expression quantification in RPKM
    -  A quick and complete annotation similar to annotations for model organisms
    -  Deliverables compliant with freely available tools and browsers: Artemis, Mauve, EBI tools, Expasy tools

RNA-Seq is the new alternative to microarrays for analyzing gene expression obtaining a quick profiling of your transcriptome.  One advantage of RNA-seq over Expression Arrays is that RNA-seq experiments are not species specific in terms that it is not necessary to design and develop a specific array for each species.

RNA-seq allows a deep knowledge of specific aspects as:

    -  Isoforms expression splice-junctions analysis
    -  Regulatory RNAs
    -  Gene expression quantification
    -  Analysis of differential expression between different samples

We work with the most advanced pipelines based in programs as Tophat and Cufflinks. We add rich functional data to your results and provide you gene ontology analysis and other complex enrichment studies requested by the client.

Contact us by e-mail:

  • info@era7.com

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