New article from Oh no sequences!, Era7 Bioinformatics' research group: Genome Sequence of Klebsiella pneumoniae KpQ3, a DHA-1 β-Lactamase-Producing Nosocomial Isolate.

Klebsiella pneumoniae is a Gram-negative bacillus that belongs to the family Enterobacteriaceae. K. pneumoniae isolates are mostly commensal and environmental microorganisms but may be opportunistic and nosocomial pathogens that cause a variety of infections, including infections of the urinary and respiratory tracts, bloodstream, and soft tissue. This bacterium has a significant capacity to acquire antibiotic resistance.

K. pneumoniae KpQ3 was isolated from a blood culture from a burn unit patient in the context of a small outbreak at Hospital Universitario La Paz. Oh no sequences team from Era7 Bioinformatics did the assembly and the annotation of the genome. Assembly was done with Newbler and manually refined. The KpQ3 genome was submitted to GenBank (AMSU00000000) with 73 contigs assembled in 18 scaffolds with a genome global length of 5,401,505 bp. Genome annotation was done using the Era7 Bioinformatics' Bacterial Genome Annotation system BG7.

This work, published in the journal Genome Announcements from the American Association of Microbiology, is a new success of Era7 Bioinformatics related to its project NEXTMICRO. The goal of this project is the development of a platform able to manage all the NGS sequence data and related data important in an outbreak. The NEXTMICRO project includes the BG7 annotation system and new Cloud Computing pipelines and services specially designed or adapted to manage NGS outbreak data from hospital and community outbreaks.